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Immunology and Infection

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JoVE Journal Immunology and Infection
Niet-chirurgische intratracheale Instillatie van Muizen met Analyse van de longen en Lung drainerende lymfeklieren door middel van flowcytometrie
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Niet-chirurgische intratracheale Instillatie van Muizen met Analyse van de longen en Lung drainerende lymfeklieren door middel van flowcytometrie

Article DOI: 10.3791/2702-v • 08:02 min • May 2nd, 2011
May 2nd, 2011 •
1, 2, 1, 3, 1,2,4, 1,4
1Department of Immunology, University of Colorado School of Medicine, 2Division of Cell Biology, Department of Pediatrics, National Jewish Health, 3Department of Microbiology, Immunology, and Pathology, Colorado State University, 4Department of Immunology, National Jewish Health

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We illustreren niet-chirurgische levering van testmateriaal in de longen van verdoofde muizen via de luchtpijp. Deze methode laat long blootstelling aan bacteriële en virale pathogenen, cytokines, antistoffen, kralen, chemicaliën of kleurstoffen. We verder beschrijven oogst en verwerking van de longen en longen drainerende lymfeklieren (LDLNs) voor flowcytometrie.

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Immunologie intratracheale muis longen long-drainerende lymfeklieren flowcytometrie
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