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JoVE Journal
Neuroscience

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Vibrodissociation从啮齿动物脑切片的神经元,研究突触传递和图像突触前的码头
 
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Vibrodissociation从啮齿动物脑切片的神经元,研究突触传递和图像突触前的码头

Article DOI: 10.3791/2752-v 08:38 min May 25th, 2011
May 25th, 2011

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Summary

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Click here for the English version.

这份报告表明保留个别可行的神经元连接的突触前boutons的机械隔离技术。 Vibrodissociated的神经元具有快速生产的优势,卓越的药理控制和改进的空间不影响邻近的细胞钳。这种方法可用于突触元素的成像和膜片钳记录。

Tags

第51期,神经科学,神经解离,突触传递,氨基丁酸,钙成像,电生理学,海马,纹状体
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