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JoVE Journal
Immunology and Infection

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高效转染树突状细胞的无细胞的成熟优化的协议
 
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高效转染树突状细胞的无细胞的成熟优化的协议

Article DOI: 10.3791/2766
July 8th, 2011

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Summary July 8th, 2011

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我们目前我们优化的高通量nucleofection协议,要么质粒DNA或不会造成细胞的成熟的siRNA转染初级人类单核细胞衍生的树突状细胞的有效方法。我们进一步提供证据证明为成功的siRNA沉默靶基因的mRNA和蛋白水平的RIG - I。

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