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Protocolo otimizado para transfecção eficiente de células dendríticas, sem maturação das células
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Protocolo otimizado para transfecção eficiente de células dendríticas, sem maturação das células

Article DOI: 10.3791/2766-v • 08:08 min • July 8th, 2011
July 8th, 2011 •
*1, *1, 1, 1
1Center for Translational Systems Biology and Department of Neurology, Mount Sinai School of Medicine
* These authors contributed equally

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Apresentamos nosso protocolo nucleofection high-throughput otimizado como uma forma eficiente de transfecting humanos primários derivados de monócitos com células dendríticas ou DNA plasmídeo ou siRNA sem causar maturação das células. Nós ainda fornecer provas para silenciar siRNA sucesso do gene alvo RIG-I em ambos os mRNA e os níveis de proteína.

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Imunologia Edição 53 as células dendríticas nucleofection high-throughput siRNA interferon de sinalização
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