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JoVE Journal
Biology

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在酵母用于监测Mitophagy荧光显微镜检测酿酒酵母
 
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在酵母用于监测Mitophagy荧光显微镜检测酿酒酵母

Article DOI: 10.3791/2779-v 10:28 min July 18th, 2011
July 18th, 2011

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Summary

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Click here for the English version.

一个强大的方法来监视传递到细胞器的降解和回收的酵母液泡酸性流明描述。该方法依赖一种基因编码的双发射荧光pH值传感器,和可视化的单个细胞,用荧光显微镜对特定目标细胞器的标签。

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细胞生物学,53期,自噬,显微镜,线粒体,细胞核,酵母
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