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JoVE Journal
Biology

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量化传播肿瘤细胞的频率使用斑马鱼的同源有限稀释细胞移植
 
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量化传播肿瘤细胞的频率使用斑马鱼的同源有限稀释细胞移植

Article DOI: 10.3791/2790-v 08:01 min July 14th, 2011
July 14th, 2011

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Summary

Please note that all translations are automatically generated.

Click here for the English version.

有限稀释细胞移植试验是用来确定传播肿瘤细胞的频率。本协议描述了一个产生,发展荧光标记的白血病和细节,如何限制稀释到成年斑马鱼的腹腔这些白血病细胞分离和移植的同源斑马鱼的方法。

Tags

发育生物学,53期,癌症干细胞,T细胞急性淋巴细胞白血病,显微注射,荧光,自我更新
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