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Journal / Immunology and Infection / Immuno-Fluoreszenz-Assay von…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Immuno-Fluoreszenz-Assay von Leptospira-Surface-exponierten Proteine
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Immuno-Fluoreszenz-Assay von Leptospira-Surface-exponierten Proteine

Article DOI: 10.3791/2805-v • 10:47 min • July 1st, 2011
July 1st, 2011 •
1,2, 3,4
1Department of Medicine, David Geffen School of Medicine, University of California, Los Angeles, 2Research service, 151, Veterans Affairs Greater Los Angeles Healthcare System, 3Departments of Medicine, Urology at David Geffen School of Medicine and Department of Microbiology, Immunology and Molecular Gentics, University of California Los Angeles (UCLA), 4Division of Infectious Diseases, 111F, Veterans Affairs Greater Los Angeles Health Care System

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Eine effiziente Methode, um Oberflächen-Exposition von Leptospira-Proteine ​​zu beurteilen ist beschrieben. Die Methode ist speziell für die Störung des empfindlichen äußeren Membran von Leptospira-Zellen zu vermeiden. Diese Technik erfordert Beschäftigung von mehreren negativen Kontrollen, um die Integrität der äußeren Membran und eine Spezifität von Antikörper-Reaktion zu beurteilen.

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Immunologie Molecular Biology Leptospira intakte Zellen äußere Membran Oberflächen-exponierten Proteine Oberfläche Immunfluoreszenz
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