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Een snelle aanpak van High-Resolution Imaging fluorescentie in Semi-Dik Brain Slices

Article DOI: 10.3791/2807-v • 04:35 min • July 26th, 2011
July 26th, 2011 •
1, 2, 3,4
1Department of Molecular & Human Genetics, Baylor College of Medicine (BCM), 2Precisionary Instruments Inc., 3Departments of Molecular & Human Genetics and Neuroscience, Baylor College of Medicine (BCM), 4Jan and Dan Duncan Neurological Research Institute, Texas Children's Hospital

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Hier beschrijven we een snelle en eenvoudige methode om de afbeelding fluorescent gelabelde cellen in semi-dikke hersenen plakken. Door de vaststelling van, snijden, en optisch clearing hersenweefsel beschrijven we hoe standaard epifluorescerende of confocale beeldvorming kan worden gebruikt om individuele cellen en neuronale netwerken binnen intact zenuwweefsel te visualiseren.

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Neurowetenschappen zenuwweefsel neuronen confocale epifluorescerende imaging clearing fluorescente eiwitten
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