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JoVE Journal Neuroscience
Un metodo rapido per High-Resolution Imaging di fluorescenza in semi-spessa fettine di cervello
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Un metodo rapido per High-Resolution Imaging di fluorescenza in semi-spessa fettine di cervello

Article DOI: 10.3791/2807-v • 04:35 min • July 26th, 2011
July 26th, 2011 •
1, 2, 3,4
1Department of Molecular & Human Genetics, Baylor College of Medicine (BCM), 2Precisionary Instruments Inc., 3Departments of Molecular & Human Genetics and Neuroscience, Baylor College of Medicine (BCM), 4Jan and Dan Duncan Neurological Research Institute, Texas Children's Hospital

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Qui si descrive un metodo rapido e semplice per celle di immagine fluorescente in semi-densi fettine di cervello. Fissando, affettare, e otticamente compensazione tessuto cerebrale si descrivono le modalità standard di imaging epifluorescente o confocale può essere utilizzato per visualizzare le singole cellule e reti neuronali all'interno intatto tessuto nervoso.

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Neuroscienze Numero 53 tessuto nervoso i neuroni confocale epifluorescente l'imaging la compensazione proteine ​​fluorescenti
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