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JoVE Journal
Biology

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加上质谱肽免疫亲和富集的蛋白质定量
 

加上质谱肽免疫亲和富集的蛋白质定量

Article doi: 10.3791/2812
July 31st, 2011

Summary July 31st, 2011

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稳定同位素标准和捕捉反肽抗体(SISCAPA)夫妇亲和富集稳定同位素稀释质谱(MRM - MS)的肽作为代理人为各自的蛋白质肽的定量测量提供。在这里,我们描述了一个部分自动化的格式使用磁性粒子的协议。

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