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Neuroscience

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JoVE Journal Neuroscience
Kvantifiera aktivitet Cis-Reglerande element i musen Retina av Explantation elektroporation
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Kvantifiera aktivitet Cis-Reglerande element i musen Retina av Explantation elektroporation

Article DOI: 10.3791/2821-v • 07:39 min • June 28th, 2011
June 28th, 2011 •
1, 1, 1
1Department of Pathology and Immunology, Washington University School of Medicine

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Detta protokoll beskriver ett enkelt och billigt sätt att kvantifiera aktiviteten hos cis-reglerande element (dvs förstärkare / projektansvariga) i levande mus näthinnor via Explantation elektroporation. DNA-preparation, retinal dissektion, elektroporation, retinal Explantation kultur, och efter fixering analys och kvantifiering beskrivs.

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Neurovetenskap näthinnan ljusmätare cis-reglerande element kvantifiering elektroporation promotor analys
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