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Screening-analys för oxidativ stress i ett felint astrocyternas Cell Line, G355-5
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Screening-analys för oxidativ stress i ett felint astrocyternas Cell Line, G355-5

Article DOI: 10.3791/2841-v • 09:53 min • July 13th, 2011
July 13th, 2011 •
1, 1, 1, 2, 3, 2, 1,2
1College of Veterinary Medicine, Western University of Health Sciences, 2Graduate College of Biomedical Sciences, Western University of Health Sciences, 3ReadiSorb, Products

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En screeningmetod för att upptäcka oxidativ cellulära miljöer är att mäta oxidation av CM-H2DCFDA. När oxideras i en cell, CM-H2DCFDA förändringar från icke-fluorescerande i en fluorescerande förening. Denna förändring i fluorescens mäts med flödescytometri och anger antalet celler i en oxidativ miljö.

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Neurovetenskap 53 astrocyter oxidativ stress flödescytometri CM-H2DCFDA
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