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Biology

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Bromodeoxyuridine (BrdU) Labeling and Subsequent Fluorescence Activated Cell Sorting for Culture-independent Identification of Dissolved Organic Carbon-degrading Bacterioplankton
 
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Bromodeoxyuridine (BrdU) Labeling and Subsequent Fluorescence Activated Cell Sorting for Culture-independent Identification of Dissolved Organic Carbon-degrading Bacterioplankton

Article DOI: 10.3791/2855-v 12:46 min September 10th, 2011
September 10th, 2011

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Summary

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Click here for the English version.

Environmental bacterioplankton are incubated with a model dissolved organic carbon (DOC) compound and a DNA labeling reagent, bromodeoxyuridine (BrdU). Afterward, DOC-degrading cells are separated from the bulk community based on their elevated BrdU incorporation using fluorescence activated cell sorting (FACS). These cells are then identified by subsequent molecular analyses.

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Bromodeoxyuridine BrdU Labeling Fluorescence Activated Cell Sorting Culture-independent Identification Dissolved Organic Carbon Bacterioplankton Microbial Degradation Aquatic Environments 16S RRNA Gene-based Molecular Analysis DOC Compounds Microcosms Immunodetection HI Cells LI Cells
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