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JoVE Journal
Biology

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溴脱氧尿嘧啶核苷(BrdU)标记和文化独立的鉴定溶解有机随后的荧光激活细胞分选碳有辱人格的浮游细菌
 
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溴脱氧尿嘧啶核苷(BrdU)标记和文化独立的鉴定溶解有机随后的荧光激活细胞分选碳有辱人格的浮游细菌

Article DOI: 10.3791/2855-v 12:46 min September 10th, 2011
September 10th, 2011

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Summary

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Click here for the English version.

环境浮游细菌培养与溶解有机碳(DOC)的化合物和一个DNA标记试剂,溴脱氧尿嘧啶核苷(BrdU)的一种模式。随后,商务部降解细胞分离升高BrdU使用荧光激活细胞分选(FACS)纳入散装社会。随后的分子分析这些细胞,然后确定。

Tags

分子生物学,55期,BrdU掺入,荧光激活细胞分选,流式细胞仪,流式细胞仪,微生物群落,文化独立,浮游细菌
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