Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biology / ReAsH / Flash Labeling und Bildanalyse von…
JoVE Journal
Biology

A subscription to JoVE is required to view this content.

JoVE Journal Biology
ReAsH / Flash Labeling und Bildanalyse von Tetracysteinmotiv Sensor Proteinen in Zellen
Microinjection of mRNA and Morpholino Antisense Oligonucleotides in Zebrafish Embryos.
Journal (Biology)
Microinjection of mRNA and Morpholino Antisense Oligonucleotides in Zebrafish Embryos.
A Protocol for Functional Assessment of Whole-Protein Saturation Mutagenesis Libraries Utilizing High-Throughput Sequencing
Journal (Biology)
A Protocol for Functional Assessment of Whole-Protein Saturation Mutagenesis Libraries Utilizing High-Throughput Sequencing
 
Click here for the English version

ReAsH / Flash Labeling und Bildanalyse von Tetracysteinmotiv Sensor Proteinen in Zellen

Article DOI: 10.3791/2857-v • 12:20 min • August 31st, 2011
August 31st, 2011 •
1, 1, 1, 1
1Department of Biochemistry and Molecular Biology, Bio21 Molecular Science and Biotechnology Institute

Chapters

Summary
Automatic Translation

Please note that all translations are automatically generated.

Click here for the English version.

Die biarsenical Farbstoffe Flash und ReAsH binden spezifisch an Tetracysteinmotiv Motive in Proteinen und kann wahlweise Label Proteine ​​in lebenden Zellen. Kürzlich diese Kennzeichnung Strategie wurde verwendet, um Sensoren für verschiedene Proteinkonformationen oder oligomeren Zustände zu entwickeln. Wir beschreiben die Label-Ansatz und Methoden zur quantitativen Analyse bindend.

Tags

Cell Biology Ausgabe 54 Tetracysteinmotiv TC ReAsH Flash biarsenical Farbstoffe Fluoreszenz- Imaging- konfokale Mikroskopie ImageJ GFP
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter