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Mesure de la cinétique de la transcription d'ARNm dans les cellules vivantes simples
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Mesure de la cinétique de la transcription d'ARNm dans les cellules vivantes simples

Article DOI: 10.3791/2898
August 25th, 2011 •
1, 1
1The Mina and Everard Goodman Faculty of Life Sciences and Institute of Nanotechnology, Bar-Ilan University

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August 25th, 2011

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L'ARN polymérase II cinétiques transcriptionnelles sont mesurés sur des gènes spécifiques dans les cellules vivantes. ARNm transcrit à partir du gène d'intérêt sont marqués par fluorescence et de l'utilisation de récupération de fluorescence après photoblanchiment (FRAP) la cinétique in vivo de l'élongation de la transcription sont obtenues.

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