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Measuring the Kinetics of mRNA Transcription in Single Living Cells

Article DOI: 10.3791/2898-v • 11:22 min • August 25th, 2011
August 25th, 2011 •
1, 1
1The Mina and Everard Goodman Faculty of Life Sciences and Institute of Nanotechnology, Bar-Ilan University

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RNA polymerase II transcriptional kinetics are measured on specific genes in living cells. mRNAs transcribed from the gene of interest are fluorescently tagged and using Fluorescence Recovery After Photobleaching (FRAP) the in vivo kinetics of transcriptional elongation are obtained.

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Kinetics MRNA Transcription Living Cells RNA Polymerase II Transcriptional Activity Visualization Methods Analysis Elongation Kinetics Gene Locus Fluorescent Labeling MS2 System MRNA Transcripts YFP-MS2 Coat Protein Fluorescence Recovery After Photobleaching (FRAP) Method Synthesis Rate
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