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Efficient Gene Delivery into Multiple CNS Territories Using In Utero Electroporation

Article DOI: 10.3791/2957-v • 13:13 min • June 23rd, 2011
June 23rd, 2011 •
1, 2, 1, 1,2, 1, 2, 1
1Department of Biochemistry and Molecular Biology, Hotchkiss Brain Institute, Alberta Children’s Hospital Research Institute, University of Calgary, 2Department of Medical Genetics, Alberta Children’s Hospital Research Institute, Hotchkiss Brain Institute, University of Calgary

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Summary
Automatic Translation

In utero electroporation allows for rapid gene delivery in a spatially- and temporally-controlled manner in the developing central nervous system (CNS). Here we describe a highly adaptable in utero electroporation protocol that can be used to deliver expression constructs into multiple embryonic CNS domains, including the telencephalon, diencephalon and retina.

Tags

Gene Delivery CNS Territories In Utero Electroporation Gene Expression Developmental Pathways Transgenic Technologies Loss-of-function Models Gain-of-function Models Mouse Transgenics Alternative Methods Live Mouse Embryos DNA Injection Electrical Current Cell Membranes Embryonic Day (E) 11.5 Ultrasound-guided Microinjection Protocol E15.5 Stage Parietal Bone Differentiation Frontal Bone Differentiation Retina
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