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Neuroscience

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JoVE Journal Neuroscience
Efficiënte gen delivery in meerdere CNS Gebieden Het gebruik van In Utero Elektroporatie
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Efficiënte gen delivery in meerdere CNS Gebieden Het gebruik van In Utero Elektroporatie

Article DOI: 10.3791/2957
June 23rd, 2011 •
1, 2, 1, 1,2, 1, 2, 1
1Department of Biochemistry and Molecular Biology, Hotchkiss Brain Institute, Alberta Children’s Hospital Research Institute, University of Calgary, 2Department of Medical Genetics, Alberta Children’s Hospital Research Institute, Hotchkiss Brain Institute, University of Calgary

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June 23rd, 2011

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In utero elektroporatie zorgt voor een snelle genafgifte in een ruimtelijk-temporeel en gecontroleerde wijze in de zich ontwikkelende centrale zenuwstelsel (CZS). Hier beschrijven we een groot aanpassingsvermogen in utero electroporatie protocol dat gebruikt kan worden om expressie constructen te leveren in meerdere embryonale CNS domeinen, waaronder de telencephalon, diencephalon en netvlies.

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