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JoVE Journal
Neuroscience

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JoVE Journal Neuroscience
Effiziente Gene Lieferung in mehrere ZNS Territories mit In Utero Elektroporation
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Effiziente Gene Lieferung in mehrere ZNS Territories mit In Utero Elektroporation

Article DOI: 10.3791/2957
June 23rd, 2011 •
1, 2, 1, 1,2, 1, 2, 1
1Department of Biochemistry and Molecular Biology, Hotchkiss Brain Institute, Alberta Children’s Hospital Research Institute, University of Calgary, 2Department of Medical Genetics, Alberta Children’s Hospital Research Institute, Hotchkiss Brain Institute, University of Calgary

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June 23rd, 2011

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In utero Elektroporation ermöglicht eine schnelle Gentransfer in eine räumlich-und zeitlich kontrollierten Art und Weise in den Entwicklungsländern das zentrale Nervensystem (ZNS). Hier beschreiben wir eine sehr anpassungsfähig in utero Elektroporation Protokoll, mit dem Ausdruck Konstrukte in mehreren embryonalen ZNS-Domänen, einschließlich der Telencephalon, Diencephalon und der Netzhaut liefern können.

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