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Journal / Biology / Beredning av kvalitet Inositol Pyrophosphates
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Beredning av kvalitet Inositol Pyrophosphates
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Beredning av kvalitet Inositol Pyrophosphates

Article DOI: 10.3791/3027-v • 10:35 min • September 3rd, 2011
September 3rd, 2011 •
1, 1, 1, 1, 1
1Medical Research Council (MRC), Cell Biology Unit and Laboratory for Molecular Cell Biology, University College London

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Inositol pyrophosphates spelar en viktig roll i mänskliga sjukdomar såsom cancer, diabetes och fetma, men det är den exakta verkningsmekanismen en tvistefråga. Bristen på kommersiellt tillgängliga inositol pyrophosphates gör detaljerade studier problematiska. Här beskriver vi ett enkelt protokoll för att producera och isolera milligram inositol pyrophosphates.

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Molekylärbiologi Polyacrilamyde gelelektrofores (sida) inositol hexakisphosphate (IP6) fytinsyra diphosphoinositol pentakisphosphate (IP7) bisdiphoshoinositol tetrakisphosphate (IP8) ip6-kinas (IP6K) PP-IP5K VIP1
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