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Journal / Bioengineering / Visualisering av Rekombinant DNA och…
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Bioengineering

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JoVE Journal Bioengineering
Visualisering av Rekombinant DNA och proteinkomplex Använda atomkraftsmikroskopi
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Visualisering av Rekombinant DNA och proteinkomplex Använda atomkraftsmikroskopi

Article DOI: 10.3791/3061-v • 08:30 min • July 18th, 2011
July 18th, 2011 •
1, 2, 2
1College of Nursing, Interdisciplinary Life Sciences Research Laboratory, Seattle University, 2College of Science and Engineering, Interdisciplinary Life Sciences Research Laboratory, Seattle University

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En knacka läge atomkraftsmikroskop (AFM) metod för visualisering av plasmid-DNA, cytoplasma proteiner och DNA-protein komplex beskrivs. Metoden innehåller alternativa metoder för att förbereda prover för AFM bildhantering följande biokemiska manipulation. DNA innehåller specifika regioner protein interagerar observeras i nära fysiologiska bufferten förhållanden.

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Bioteknik atomkraftsmikroskopi glukokortikoidreceptorn protein-protein växelverkan DNA-protein växelverkan svepspetsmikroskopi immunoadsorption
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