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Detectie van histon Wijzigingen in bladeren
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Detectie van histon Wijzigingen in bladeren

Article DOI: 10.3791/3096-v • 07:09 min • September 23rd, 2011
September 23rd, 2011 •
1,2, 3, 2
1Department of Botany, RWTH Aachen University, 2Department of Plant Physiology, RWTH Aachen University, 3Department of Botany, Leibniz University

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Een betrouwbare en bruikbare benadering van de histon-modificaties op specifieke plantengenen te detecteren is beschreven. De aanpak combineert chromatine immunoprecipitatie (chip) en real-time kwantitatieve PCR. Het maakt de ontdekking van histon-modificaties op specifieke genen met een rol spelen in verschillende fysiologische processen.

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Moleculaire Biologie chromatine chromatine immunoprecipitatie chip histon-modificaties PCR planten moleculaire biologie plant promotor controle genregulatie
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