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Journal / Immunology and Infection / L'uso di una trappola ottica per lo Studio…
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Immunology and Infection

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JoVE Journal Immunology and Infection
L'uso di una trappola ottica per lo Studio delle interazioni ospite-patogeno per Dynamic Imaging Live Cell
Simultaneous Study of the Recruitment of Monocyte Subpopulations Under Flow In Vitro
Journal (Immunology and Infection)
Simultaneous Study of the Recruitment of Monocyte Subpopulations Under Flow In Vitro
Qualitative and Quantitative Analysis of the Immune Synapse in the Human System Using Imaging Flow Cytometry
Journal (Immunology and Infection)
Qualitative and Quantitative Analysis of the Immune Synapse in the Human System Using Imaging Flow Cytometry
 
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L'uso di una trappola ottica per lo Studio delle interazioni ospite-patogeno per Dynamic Imaging Live Cell

Article DOI: 10.3791/3123-v • 09:17 min • July 28th, 2011
July 28th, 2011 •
1, 2, 3, 1, 1, 3, 4, 1
1Department of Medicine, Division of Infectious Diseases, Massachusetts General Hospital, Harvard Medical School, 2Department of Mechanical and Aerospace Engineering, The Ohio State University, 3Center for Computational and Integrative Biology, Massachusetts General Hospital, Harvard Medical School, 4Dept. of Chemical and Biomolecular Engineering, Vanderbilt University

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Un metodo è descritto per selezionare individualmente, manipolare e agenti patogeni immagine dal vivo utilizzando una trappola ottica accoppiato ad un microscopio a disco rotante. La trappola ottica fornisce il controllo spaziale e temporale di organismi e li adiacenti alle cellule ospite. Microscopia a fluorescenza cattura le interazioni intercellulare perturbazione con minimo di cellule.

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Immunologia Numero 53 intrappolamento ottico pinzette ottiche delle cellule T patogeno l'imaging cellulare dal vivo la microscopia confocale disco rotante Aspergillus fumigatus Candida albicans funghi
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