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JoVE Journal Neuroscience
Profiling Voltage-gated Potassium Channel-mRNA Expression in nigralen Neuronen mit Einzel-Zell RT-PCR-Techniken
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Profiling Voltage-gated Potassium Channel-mRNA Expression in nigralen Neuronen mit Einzel-Zell RT-PCR-Techniken

Article DOI: 10.3791/3136-v • 07:31 min • September 27th, 2011
September 27th, 2011 •
1, 1
1Department of Pharmacology, University of Tennessee College of Medicine

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Neuronen sind zunächst geprägt elektrophysiologisch. Dann das Zytoplasma aus den aufgezeichneten Neuronen wird abgesaugt und einer Transkription-PCR-Analyse umzukehren, um die Expression von mRNAs für Neurotransmitter-Synthese-Enzyme, Ionenkanäle und Rezeptoren zu erkennen.

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Neuroscience Ausgabe 55 Aktionspotential mRNA Patch-Clamp- Einzel-Zell RT-PCR PCR Substantia nigra
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