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Neuroscience

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JoVE Journal Neuroscience
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Quantitative Analysis of Synaptic Vesicle Pool Replenishment in Cultured Cerebellar Granule Neurons using FM Dyes

Article DOI: 10.3791/3143-v • 09:02 min • November 11th, 2011
November 11th, 2011 •
1, 1
1Membrane Biology Group, Centre for integrative Physiology, University of Edinburgh

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Summary
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A live fluorescence imaging technique to quantify the replenishment and mobilisation of specific synaptic vesicle (SV) pools in central nerve terminals is described. Two rounds of SV recycling are monitored in the same nerve terminals providing an internal control.

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Quantitative Analysis Synaptic Vesicle Pool Replenishment Cultured Cerebellar Granule Neurons FM Dyes Neurotransmitter Release Endocytosis Recycling Pool Readily Releasable Pool Reserve Pool Assay SV Traffic Clathrin-dependent Endocytosis Activity-dependent Bulk Endocytosis Mobilisation Mechanisms SV Turnover Central Nerve Terminals Hydrophobic Hydrocarbon Tail Hydrophilic Head Group Fluorescent Probes
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