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Journal / Biology / A Quantitative Assay für Insulin-exprimierenden…
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Biology

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A Quantitative Assay für Insulin-exprimierenden Koloniebildende Vorläufern
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A Quantitative Assay für Insulin-exprimierenden Koloniebildende Vorläufern

Article DOI: 10.3791/3148-v • 14:39 min • November 28th, 2011
November 28th, 2011 •
1, 2, 2, 2, 2, 2, 2,3
1Department of Biotechnology & Bioinformatics, California State University Channel Islands, 2Department of Diabetes, Endocrinology and Metabolism, Beckman Research Institute of City of Hope, 3The Irell & Manella Graduate School of Biological Sciences, Beckman Research Institute of City of Hope

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Eine dreidimensionale klonogene Test, dass Pankreas-like Vorläuferzellen in Insulin-exprimierenden Kolonien unterscheiden können beschrieben. Diese Methode nutzt die Vorteile von halbfesten Medien mit Methylcellulose, Matrigel und Wachstumsfaktoren, in denen einzelne Vorläuferzellen proliferieren und differenzieren

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Developmental Biology Ausgabe 57 Bauchspeicheldrüse Insulin-exprimierenden Zellen embryonale Stammzellen Kolonie-Assay Vorläuferzellen 3-dimensionale Kultur halbfeste Medien Matrigel Methylcellulose
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