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Uma análise quantitativa para expressar Insulin-formadoras de colônias Progenitores
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Uma análise quantitativa para expressar Insulin-formadoras de colônias Progenitores

Article DOI: 10.3791/3148-v • 14:39 min • November 28th, 2011
November 28th, 2011 •
1, 2, 2, 2, 2, 2, 2,3
1Department of Biotechnology & Bioinformatics, California State University Channel Islands, 2Department of Diabetes, Endocrinology and Metabolism, Beckman Research Institute of City of Hope, 3The Irell & Manella Graduate School of Biological Sciences, Beckman Research Institute of City of Hope

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A tridimensionais ensaio clonogênica que permite pancreático-como progenitores de se diferenciar em insulina expressar colônias é descrito. Este método tira vantagem de semi-sólido mídia contendo fatores Matrigel metilcelulose, e crescimento, no qual progenitores única proliferam e se diferenciam

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Biologia do Desenvolvimento Pâncreas células que expressam insulina as células-tronco embrionárias ensaio de colônia as células progenitoras 3-dimensional cultura semi-sólido mídia Matrigel metilcelulose
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