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Journal / Biology / DNA-methylatie: bisulfiet Wijziging en Analyse
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Biology

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JoVE Journal Biology
DNA-methylatie
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DNA-methylatie: bisulfiet Wijziging en Analyse

Article DOI: 10.3791/3170-v • 12:34 min • October 21st, 2011
October 21st, 2011 •
*1, *1, 1, 1,2
1Epigenetics Group, Cancer Research Program, Garvan Institute of Medical Research, 2St Vincent's Clinical School, University of NSW
* These authors contributed equally

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De gouden standaard voor DNA methylatie analyse is genomische sequentie van bisulfiet omgezet DNA. Deze methode maakt gebruik van de verhoogde gevoeligheid van cytosine in vergelijking met 5-methylcytosine (5-MEC) tegen desaminering bisulfiet onder zure omstandigheden. Niet-gemethyleerd cytosines kunnen worden onderscheiden van gemethyleerd cytosines na PCR-amplificatie van het doel genomisch DNA.

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Genetica epigenetica DNA methylatie bisulfiet 5-methylcytosine (5-MEC) PCR
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