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Journal / Biology / DNA methylering: bisulfit Ændring og analyse
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DNA methylering
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DNA methylering: bisulfit Ændring og analyse

Article DOI: 10.3791/3170-v • 12:34 min • October 21st, 2011
October 21st, 2011 •
*1, *1, 1, 1,2
1Epigenetics Group, Cancer Research Program, Garvan Institute of Medical Research, 2St Vincent's Clinical School, University of NSW
* These authors contributed equally

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Den gyldne standard for DNA methylering analyse er genomiske sekventering af bisulfit konverteret DNA. Denne metode benytter sig af den øgede følsomhed cytosin sammenlignet med 5-methylcytosine (5-MEC) til bisulfit deaminering under sure betingelser. Unmethylated cytosines kan skelnes fra methyleres cytosines efter PCR-amplifikation af målet genomisk DNA.

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Genetik epigenetik DNA methylering bisulfit 5-methylcytosine (5-MEC) PCR
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