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JoVE Journal
Biology

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DNA methylering
 
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DNA methylering: bisulfit Ændring og analyse

Article DOI: 10.3791/3170
October 21st, 2011

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Summary October 21st, 2011

Please note that all translations are automatically generated.

Click here for the English version.

Den gyldne standard for DNA methylering analyse er genomiske sekventering af bisulfit konverteret DNA. Denne metode benytter sig af den øgede følsomhed cytosin sammenlignet med 5-methylcytosine (5-MEC) til bisulfit deaminering under sure betingelser. Unmethylated cytosines kan skelnes fra methyleres cytosines efter PCR-amplifikation af målet genomisk DNA.

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