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Journal / Biology / Metilazione del DNA: Modifica bisolfito e analisi
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Biology

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JoVE Journal Biology
Metilazione del DNA
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Metilazione del DNA: Modifica bisolfito e analisi

Article DOI: 10.3791/3170-v • 12:34 min • October 21st, 2011
October 21st, 2011 •
*1, *1, 1, 1,2
1Epigenetics Group, Cancer Research Program, Garvan Institute of Medical Research, 2St Vincent's Clinical School, University of NSW
* These authors contributed equally

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Il gold standard per l'analisi di metilazione del DNA è il sequenziamento del DNA genomico di bisolfito convertito. Questo metodo sfrutta l'accresciuta sensibilità della citosina rispetto ai 5-metilcitosina (5-MEC) di bisolfito deaminazione in condizioni acide. Cytosines non metilato può essere distinto dal cytosines metilato dopo amplificazione del DNA genomico di destinazione.

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Genetica Numero 56 l'epigenetica la metilazione del DNA bisolfito 5-metilcitosina (5-MEC) PCR
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