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Journal / Biology / Encelliga Fate Kartläggning i zebrafisk
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Biology

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Encelliga Fate Kartläggning i zebrafisk

Article DOI: 10.3791/3172-v • 07:53 min • October 5th, 2011
October 5th, 2011 •
1, 1, 1,2
1Division of Developmental Biology, Cincinnati Children's Hospital Medical Center, 2Division of Hematology/Oncology, Cincinnati Children's Hospital Medical Center

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En metod beskrivs för att photoactivate enskilda celler innehållande en bur fluorescerande protein med två-foton-absorption från en Ti: Sapphire femtosecond laseroscillatorn. För ödet karta fotoaktiverad cell, immunohistokemi används. Denna teknik kan tillämpas på varje celltyp.

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Utvecklingsbiologi zebrafisk två-photon fotoaktivering immunohistokemi caged fluorescerande protein öde kartläggning
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