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Isolering av CD133 Stem + leverceller för klonal expansion
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Isolering av CD133 Stem + leverceller för klonal expansion

Article DOI: 10.3791/3183-v • 12:07 min • October 10th, 2011
October 10th, 2011 •
1, 1, 1, 2, 3
1Department of Pediatrics and Pharmacology, Pennsylvania State College of Medicine, 2Department of Pharmacology, Pennsylvania State College of Medicine, 3Department of Pediatrics, University of California Los Angeles, School of Medicine

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Här beskriver vi isolering av CD133 uttrycker leverstamceller och cancer stamceller från hela murina lever, en process som kräver vävnads matsmältning, celler anrikning och flödescytometri isolering. Vi inkluderar metoder för avancerad enda cell isolering och klonal expansion.

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Utvecklingsbiologi CD133 lever stamceller oval cell levercancer stamceller stamceller celler isolering icke-parenkymal fraktion av lever flödescytometri
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