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JoVE Journal Neuroscience
Preparación de Synaptoneurosomes de ratón con una corteza discontinua de Percoll-sacarosa gradiente de densidad
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Preparación de Synaptoneurosomes de ratón con una corteza discontinua de Percoll-sacarosa gradiente de densidad

Article DOI: 10.3791/3196-v • 08:31 min • September 17th, 2011
September 17th, 2011 •
1, 1, 2, 1
1Department of Pathology and Laboratory Medicine, Waisman Center for Developmental Disabilities, University of Wisconsin, 2Department of Biochemistry, Waisman Center for Developmental Disabilities, University of Wisconsin

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Un método para preparar translationally activa, synaptoneurosomes intacta (SN) de la corteza cerebral del ratón se describe. El método utiliza una discontinua de Percoll-sacarosa gradiente de densidad que permite la rápida preparación de los ganglios centinela activa.

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Neurociencia Número 55 synaptoneurosomes sinaptosomas sacarosa Percoll-gradientes las neuronas sinapsis la corteza el ratón
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