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Preparação de Synaptoneurosomes de rato Cortex usando um gradiente de densidade Percoll descontínuo Sacarose-
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Preparação de Synaptoneurosomes de rato Cortex usando um gradiente de densidade Percoll descontínuo Sacarose-

Article DOI: 10.3791/3196-v • 08:31 min • September 17th, 2011
September 17th, 2011 •
1, 1, 2, 1
1Department of Pathology and Laboratory Medicine, Waisman Center for Developmental Disabilities, University of Wisconsin, 2Department of Biochemistry, Waisman Center for Developmental Disabilities, University of Wisconsin

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Um método para preparar traducionalmente ativa, synaptoneurosomes intacta (SNs) de córtex cerebral de rato é descrito. O método utiliza um gradiente de densidade descontínua Percoll-sacarose permitindo a preparação rápida de ativos SNs.

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Neurociência Edição 55 synaptoneurosomes sinaptossomas Percoll-sacarose gradientes os neurônios sinapse córtex mouse
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