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Journal / Neuroscience / Lektin-baserade isolering och odling av mus…
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Neuroscience

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JoVE Journal Neuroscience
Lektin-baserade isolering och odling av mus Embryonala Motoneurons
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Lektin-baserade isolering och odling av mus Embryonala Motoneurons

Article DOI: 10.3791/3200-v • 09:25 min • September 15th, 2011
September 15th, 2011 •
1, 2, 1, 2, 1, 1
1Institute for Cellmorphology and molecular Neurobiology, Group for Cellbiology, Ruhr-University Bochum, 2Institute for Clinical Neurobiology, University of Wuerzburg

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Ett alternativt sätt att isolera möss embryonala motoneurons från ryggmärgen beskrivs. Metoden tar hänsyn till det faktum att lektin kan binda till låga affinitet nerve growth factor receptor p75NTR. Denna lektin-baserade preplating ger en reningsanläggning som liknar den som med en specifik antikropp mot p75NTR.

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Neurovetenskap p75NTR ryggmärg lectin Axon Dendrite
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