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Generazione sperimentale di carcinoma-Associated fibroblasti (CAF) da fibroblasti umani mammari
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Generazione sperimentale di carcinoma-Associated fibroblasti (CAF) da fibroblasti umani mammari

Article DOI: 10.3791/3201-v • 15:43 min • October 25th, 2011
October 25th, 2011 •
*1, *1, 1,2
1CR-UK Stromal-Tumour Interaction Group, Paterson Institute for Cancer Research, University of Manchester, 2Atopy Research Center, Juntendo University
* These authors contributed equally

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Carcinoma associato fibroblasti (CAF), ricco di miofibroblasti presenti nello stroma del tumore, svolgono un ruolo importante nel guidare la progressione del tumore. Abbiamo sviluppato un tumore modello coimplantation xengraft per generare sperimentalmente CAF da fibroblasti umani mammaria. Il protocollo descrive come stabilire miofibroblasti CAF che acquisiscono la capacità di promuovere la tumorigenesi.

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Medicina Numero 56 cancro miofibroblasti stromali sperimentalmente generato carcinoma associato fibroblasti (exp-CAF) fibroblasti carcinoma mammario umano xenotrapianti tumorali
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