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使用数字免疫荧光显微镜成像在大鼠软脑膜小动脉雌激素受体α
JoVE Journal
Biology
This content is Free Access.
JoVE Journal
Biology
Imaging of Estrogen Receptor-α in Rat Pial Arterioles using a Digital Immunofluorescent Microscope
Please note that all translations are automatically generated.
Click here for the English version.
使用数字免疫荧光显微镜成像在大鼠软脑膜小动脉雌激素受体α
DOI:
10.3791/3203-v
•
07:42 min
•
November 29, 2011
•
Niloofar Rezvani
,
Andrei V. Blokhin
,
Emil Zeynalov
,
Marguerite T. Littleton-Kearney
1
Graduate School of Nursing
,
Uniformed Services University of the Health Sciences
Chapters
00:05
Title
01:42
Isolation and Preparation of Pial Arteries
03:24
ER-α Immunofluorescent Staining: Day 1
04:36
ER-α Immunofluorescent Staining: Day 2
05:30
Digital Fluorescence Imaging
06:13
Immunofluorescent ER-α Staining with Negative Controls
07:03
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
这篇文章的目的是证明的方法,优化免疫检测雌激素受体-α(ERα)使用数字免疫荧光显微镜在大鼠的软脑膜动脉切片。
Tags
Estrogen Receptor-alpha
Rat Pial Arterioles
Digital Immunofluorescent Microscope
Estrogen Receptors
Fluorescent Staining
Confocal Laser Scanning Microscopy
ER-alpha
Smooth Muscle
Endothelial Cells
Rat Basilar Arteries
Immunohistochemical Techniques
Cross-sectional Slices
Pial Arteries
Cryostat
Vessel Planes
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