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Bioengineering
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心肌在心脏组织工程中的纤维蛋白凝胶的封装
JoVE Journal
Bioengineering
This content is Free Access.
JoVE Journal
Bioengineering
Encapsulation of Cardiomyocytes in a Fibrin Hydrogel for Cardiac Tissue Engineering
Please note that all translations are automatically generated.
Click here for the English version.
心肌在心脏组织工程中的纤维蛋白凝胶的封装
DOI:
10.3791/3251-v
•
10:18 min
•
September 19, 2011
•
Kathy Yuan Ye
,
Kelly Elizabeth Sullivan
,
Lauren Deems Black
1
Department of Biomedical Engineering
,
Tufts University
Chapters
00:05
Title
01:26
Neonatal Cardiomyocyte Isolation
03:45
Casting Fibrin Gel Constructs via Injection Molding
06:56
Contraction Force Testing
08:17
Representative Fibrin Construct Analyses
09:15
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
我们描述的隔离乳鼠心肌细胞,并准备在组织工程中的纤维蛋白凝胶结构的封装的细胞。我们描述了分析后,电刺激和细胞活力,免疫组织化学染色后产生的积极力量的文化时期,包括组织工程化心肌的方法。
Tags
Encapsulation
Cardiomyocytes
Fibrin Hydrogel
Three Dimensional Hydrogel
Culture Conditions
Synthetic Polymers
PEG-DA
PLGA
Naturally Occurring Proteins
Collagen
Hyaluronic Acid
Fibrin
Polymerize
Mesh
Wound Healing Processes
Cell-degradable
Autologous
Temporary Scaffold
Isolation Of Neonatal Cardiomyocytes
Rat Pups
Preparation Of Cells
Encapsulation In Fibrin Hydrogel Constructs
In Vitro Studies
Fibrinogen
Thrombin
Fibrinopeptides FpA And FpB
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