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Neuroscience

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JoVE Journal Neuroscience
Bilaminar Co-kultur Primary Rat Kortikal neuroner og glia
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Journal (Neuroscience)
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Bilaminar Co-kultur Primary Rat Kortikal neuroner og glia

Article DOI: 10.3791/3257-v • 12:33 min • November 12th, 2011
November 12th, 2011 •
*1, *1, 1
1Department of Pharmacology and Physiology, Drexel University College of Medicine
* These authors contributed equally

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Her giver vi en protokol til dyrkning rotte cortical neuroner i overværelse af en glial feeder lag. De dyrkede neuroner etablere polaritet og skabe synapser, og kan adskilles fra den glia til brug i forskellige applikationer, såsom elektrofysiologi, calcium imaging, celle overlevelse assays, immuncytokemi, og RNA / DNA / protein isolation.

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Neurovidenskab neuron rotte hjerne co-kultur cortex glia gliaceller
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