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Journal / Neuroscience / Imaging Pheromone Sensing i en mus Vomeronasal…
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Neuroscience

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JoVE Journal Neuroscience
Imaging Pheromone Sensing i en mus Vomeronasal Akut Tissue Slice Forberedelse
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Imaging Pheromone Sensing i en mus Vomeronasal Akut Tissue Slice Forberedelse

Article DOI: 10.3791/3311-v • 09:31 min • December 6th, 2011
December 6th, 2011 •
1, 1, 1, 2, 1
1Department of Pharmacology and Toxicology, University of Lausanne, 2Department of Genetics and Evolution, University of Geneva

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Hos mus, er evnen til at detektere feromoner hovedsageligt medieres af vomeronasal organ (VNO). Her er en akut væv skive forberedelse af VNO for udførelsen af ​​calcium imaging beskrevet. Denne fysiologiske tilgang giver mulighed for observationer af delpopulationer og / eller individuelle neuroner i et levende væv og er praktisk for receptor-ligand identifikation.

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Neurovidenskab Vomeronasal orgel VNO feromon calcium imaging vævs-skive forberedelse flydende immunhistokemi GFP
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