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Journal / Immunology and Infection / 大肠癌细胞表面蛋白的分析，使用一种抗体的芯片和荧光多路复用…
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Immunology and Infection
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JoVE Journal Immunology and Infection
大肠癌细胞表面蛋白的分析，使用一种抗体的芯片和荧光多路复用
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大肠癌细胞表面蛋白的分析，使用一种抗体的芯片和荧光多路复用

Article DOI: 10.3791/3322-v • 15:18 min • September 25th, 2011
September 25th, 2011 •
1, 1, 2, 3, 4, 1
1School of Molecular Bioscience, University of Sydney, 2Department of Surgery, Royal Prince Alfred Hospital, 3Department of Anatomical Pathology, Department of Anatomical Pathology, 4Department of Medicine, Concord Repatriation General Hospital

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我们描述了一个过程分解大肠直肠癌（CRC）来产生可行的单个细胞，然后在定制的抗体，认识表面抗原（DotScan CRC芯片）芯片捕获。绑定到芯片的细胞亚群可以分析使用荧光标记的单克隆抗体与荧光染料的复用。

Tags

免疫学，第55期，大肠癌，白细胞，抗体芯片，复用，荧光，CD抗原
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