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Journal / Immunology and Infection / Colorectal Cancer Cell oppervlakte-eiwit…
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Immunology and Infection
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Colorectal Cancer Cell oppervlakte-eiwit Profiling Met behulp van een antilichaam Microarray en fluorescentie Multiplexing

Article DOI: 10.3791/3322-v • 15:18 min • September 25th, 2011
September 25th, 2011 •
1, 1, 2, 3, 4, 1
1School of Molecular Bioscience, University of Sydney, 2Department of Surgery, Royal Prince Alfred Hospital, 3Department of Anatomical Pathology, Department of Anatomical Pathology, 4Department of Medicine, Concord Repatriation General Hospital

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Beschreven wij een procedure voor het uiteenvallen van colorectale kanker (CRC) levensvatbare enkele cellen, die vervolgens worden opgevangen op maat antilichaam microarrays herkennen van oppervlakte-antigenen (DotScan CRC microarray) te produceren. Subpopulaties van cellen gebonden aan de microarray kan worden geprofileerd door fluorescentie multiplexen behulp van monoklonale antilichamen gelabeld met een fluorescerende kleurstoffen.

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Immunologie colorectale kanker leukocyten antilichaam microarray multiplexing fluorescentie CD-antigenen
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