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Journal / Immunology and Infection / 항체 Microarray 및 형광 멀티플렉싱을 사용하여 대장암 세포 표면 단백질…
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Immunology and Infection
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JoVE Journal Immunology and Infection
항체 Microarray 및 형광 멀티플렉싱을 사용하여 대장암 세포 표면 단백질 프로파일
Single-cell Analysis of Immunophenotype and Cytokine Production in Peripheral Whole Blood via Mass Cytometry
Journal (Immunology and Infection)
Single-cell Analysis of Immunophenotype and Cytokine Production in Peripheral Whole Blood via Mass Cytometry
Identifying DNA Mutations in Purified Hematopoietic Stem/Progenitor Cells
Journal (Immunology and Infection)
Identifying DNA Mutations in Purified Hematopoietic Stem/Progenitor Cells
 
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항체 Microarray 및 형광 멀티플렉싱을 사용하여 대장암 세포 표면 단백질 프로파일

Article DOI: 10.3791/3322-v • 15:18 min • September 25th, 2011
September 25th, 2011 •
1, 1, 2, 3, 4, 1
1School of Molecular Bioscience, University of Sydney, 2Department of Surgery, Royal Prince Alfred Hospital, 3Department of Anatomical Pathology, Department of Anatomical Pathology, 4Department of Medicine, Concord Repatriation General Hospital

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그러면 표면 항원 (DotScan CRC microarray)를 인식 맞춤 항체 microarrays에 캡처되어 가능한 단일 세포를 생산하는 대장암 (CRC)의 disaggregation에 대한 절차를 설명했다. microarray에 바인딩된 세포의 하위 인구는 형​​광 염료와 태그 단클론 항체를 사용하여 형광 멀티플렉싱하여 프로파일 수 있습니다.

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면역학 이슈 55 대장암 leukocytes 항체 microarray 멀티플렉싱 형광 CD의 항원
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