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Klumpformet Assay til måling af TGF-β-induceret Invasion

Article DOI: 10.3791/3337 • 09:19 min
November 16th, 2011 •
1, 1, 1, 1
1Department of Molecular Cell Biology and Centre for Biomedial Genetics, Leiden University Medical Centre

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November 16th, 2011

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En analyse til kvantitativ måling Omdannelse vækstfaktor (TGF)-β-induceret invasion i 3-dimensionelle kollagen geler er beskrevet. Denne analyse tager fordel af MCF10A serie af cellelinier, der repræsenterer forskellige stadier af brystkræft udvikling. Denne metode kan vedtages skal bruges sammen med andre cellelinjer og kan bruges til at undersøge andre potentielle aktivatorer eller inhibitorer af invasion.

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