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Leia única e emparelhados End mRNA-Seq Bibliotecas Illumina de 10 nanogramas RNA total
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Leia única e emparelhados End mRNA-Seq Bibliotecas Illumina de 10 nanogramas RNA total

Article DOI: 10.3791/3340-v • 14:50 min • October 27th, 2011
October 27th, 2011 •
1, 1, 1, 1, 1,2,3, 1, 1
1Regenerative Biology, Morgridge Institute for Research, 2Department of Cell & Regenerative Biology, University of Wisconsin, 3Department of Molecular, Cellular, & Regenerative Biology, University of California

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Aqui nós descrevemos um método para a preparação de ambos única leitura e emparelhado final Illumina mRNA Seq-libraries seqüenciamento para análise de expressão gênica com base na amplificação T7 RNA linear. Este protocolo requer apenas 10 nanogramas de começar RNA total e gera bibliotecas altamente consistentes representando transcrições todo.

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Biologia Molecular Genética mRNA-Seq Illumina-Seq perfil de expressão gênica seqüenciamento de alto rendimento
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