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JoVE Journal
Biology

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模仿人类小分子RNA的LightSwitch萤光素酶检测系统使用3'UTR记者构造的microRNA识别目标在贴壁细胞
 
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模仿人类小分子RNA的LightSwitch萤光素酶检测系统使用3'UTR记者构造的microRNA识别目标在贴壁细胞

Article DOI: 10.3791/3343
September 28th, 2011

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Summary September 28th, 2011

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Click here for the English version.

微RNA(miRNA)是基因表达的重要调节因子,已被证明在许多生物过程中发挥的作用。为了更好地了解miRNA的UTR区的相互作用,我们已经创建了一个全基因组的3 UTR的荧光素酶记者的集合,搭配了一种新的荧光素酶基因的检测试剂,LightSwitch系统。

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