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Journal / Bioengineering / Parallel-plade Flow Afdeling og Continuous Flow…
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Bioengineering

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JoVE Journal Bioengineering
Parallel-plade Flow Afdeling og Continuous Flow Circuit at evaluere endoteliale stamceller under Laminar Flow forskydningsspænding
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Parallel-plade Flow Afdeling og Continuous Flow Circuit at evaluere endoteliale stamceller under Laminar Flow forskydningsspænding

Article DOI: 10.3791/3349-v • 12:43 min • January 17th, 2012
January 17th, 2012 •
1, 2, 2, 3, 1, 1, 2, 2, 1, 4, 2, 1
1Department of Surgery, Duke University Medical Center, 2Department of Biomedical Engineering, Duke University, 3School of Medicine, University of Pennsylvania, 4Department of Medicine, Division of Cardiology, Duke University Medical Center

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Vi beskriver en metode til at underkaste tilhænger celler til laminar flow shear stress i en steril kontinuerlig strøm kredsløb. Cellerne "vedhæftning, kan morfologi undersøges gennem den gennemsigtige kammeret, prøver fra kredsløbet for metabolit analyse og celler høstes efter forskydning eksponering for fremtidige eksperimenter eller kultur.

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Bioteknik Fluid forskydningsspænding forskydningsspænding forskydningskraft endothelium endoteliale stamceller Flow Afdeling Laminar Flow Flow Circuit Continuous Flow celleadhæsionsmolekyle
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