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Bioengineering

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JoVE Journal Bioengineering
Parallel-Platten-Flow-Kammer und Continuous Flow Circuit endothelialen Vorläuferzellen unter Laminar Flow Schubspannung Bewerten
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Parallel-Platten-Flow-Kammer und Continuous Flow Circuit endothelialen Vorläuferzellen unter Laminar Flow Schubspannung Bewerten

Article DOI: 10.3791/3349-v • 12:43 min • January 17th, 2012
January 17th, 2012 •
1, 2, 2, 3, 1, 1, 2, 2, 1, 4, 2, 1
1Department of Surgery, Duke University Medical Center, 2Department of Biomedical Engineering, Duke University, 3School of Medicine, University of Pennsylvania, 4Department of Medicine, Division of Cardiology, Duke University Medical Center

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Wir beschreiben eine Methode, um unter adhärenten Zellen eine laminare Strömung Schubspannung in einem sterilen kontinuierlichen Kreislauf. Die Zellen "Haftung kann Morphologie durch die transparente Kammer untersucht werden, Proben aus der Schaltung für Metabolit-Analyse und-Zellen nach Scher-Belastung für zukünftige Experimente oder Kultur geerntet wurden.

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Bioengineering Schubspannung Schubspannung Shear Force Endothel endothelialen Vorläuferzellen Flow Chamber Laminar Flow Flow Circuit Continuous Flow Zelladhäsion
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