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JoVE Journal
Neuroscience

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Organotypiske Slice Kulturer af Embryonale ventrale midthjernen
 
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Organotypiske Slice Kulturer af Embryonale ventrale midthjernen: et system til undersøgelse Dopaminerge Neuronal Udvikling In vitro

Article DOI: 10.3791/3350-v 07:34 min January 31st, 2012
January 31st, 2012

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Summary

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En metode til at generere organotypiske skiver fra E12.5 murine embryonale midthjernen er beskrevet. Den organotypiske skive kulturer kan bruges til at observere opførslen af ​​dopaminerge neuroner eller andre ventral midthjernen neuroner.

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Neurovidenskab Developmental Biology organotypiske skive kultur midthjernen mus time-lapse imaging neuroner
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